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. 2017 Mar 8;292(16):6657–6666. doi: 10.1074/jbc.M116.755538

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 6. — CNPYb^C183A phenocopies the C39A mutant. A, gp93^Flag expressing gp96-deficient pre-B cells were further transduced with CNPYbC183A. CNPYb transgene expression was verified by improved GFP expression analyzed via flow cytometry. B, lysates from the indicated cells were resolved on a SDS-PAGE and blotted for Flag, Myc, and β-actin. Numbers denote kilodaltons. C, TLR2 and TLR4 expression was analyzed via flow cytometry of the indicated cells. Shaded histograms indicate isotype control staining.