Figure 2.

Amino acid sequence alignment of six structurally characterized eukaryotic sterol 14α-demethylases. The secondary structural elements of C. albicans (3tz1) and T. brucei (3g1q) enzymes are indicated above and below the sequences, respectively. The 34 residues invariant across the whole CYP51 family are marked with blue triangles. CYP51 family signature motifs (signature 1 and signature 2) are underscored (blue line). Black rectangles mark two phyla-specific segments: αG (protozoa) and β5 hairpin (fungi). Sequence alignment was generated in ClustalW, and secondary structural information was added in ESPript.