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Enzymatic activity of C. albicans CYP51. A, time course of lanosterol conversion (37 °C, 0.5 μm P450, 1 μm CPR, 25 μm lanosterol). Inset, HPLC profile of sterols extracted after a 1.5-min reaction. B, steady-state kinetics (1-min reaction). The experiments were performed in triplicate, and the results are presented as means ± S.E.
