Figure 3.

Arrhenius plots of the rates of RNA cleavage in the TEC-2 for WT (left) and ΔTL (right) E. coli, D. radiodurans, and T. aquaticus RNAPs. The reactions were performed at 4 different temperatures (10, 20, 30, and 37 °C for E. coli and D. radiodurans RNAPs or 40 °C for TaqRNAP) in transcription buffer containing 40 mm Tris-HCl (pH 7.9 at 25 °C), 40 mm KCl, and 10 mm MgCl₂. The plots show the dependence of the logarithm of reaction rates on reverse absolute temperature (log(k_obs) versus T⁻¹) (see supplemental Table S1 for k_obs values). The Arrhenius equation is shown on the upper right.