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Effect of CaM on the cytochrome c reduction by various cross-linked and reduced CLSS proteins. Each fully reduced CLSS that was cross-linked as indicated in each panel was mixed with excess cytochrome c at 10 °C under anaerobic conditions, and the reduction was monitored versus time at 550 nm. Horizontal and vertical dashed lines indicate the absorbance change and time elapsed during the reduction of 1 molar eq of cytochrome c by each protein. Data are representative of 5–7 trials.
