Figure 4.

Steady-state cytochrome c reductase activities versus domain cross-linker length. Solid lines, CLSS protein was cross-linked with either a disulfide bond (left-most data points) or with bismaleimides with the indicated maximum lengths, and their activities were measured at 25 °C and in the presence (■) or absence (□) of CaM. Dotted and dashed lines, activities of DTT-treated CLSS or nNOSr in the presence or absence of CaM. Values are the mean ± S.D. of three measurements, representative of three trials.