Table 3.

Equilibrium and kinetic values derived from the stopped-flow absorbance traces of cytochrome c reduction by the free or cross-linked CLSS proteins

The reactions mixed each NADPH-bound, pre-reduced enzyme with excess cytochrome c. Fitting was done using the kinetic model of Fig. 8. See text and under “Experimental procedures” for details. NA means not applicable.

Enzyme	Conformational Keq (open/closed)	Open %	k1	k−1	k3	k−3	k2
			s−1	s−1	s−1	s−1	s−1
CLSS, pH9	NA	<1	NA	NA	NA	NA	100
CLSS-BMOE	0.02	2	100	2	100	2	85
CLSS-BMB	0.15	13	80	12	80	12	65
CLSS-BMH	0.26	21	60	16	60	16	48
CLSS-BM(PEG)2	0.69	41	40	27	40	27	40
CLSS-BM(PEG)3	1.1	53	30	33	30	33	35
CLSS-BM(PEG)11	1.6	61	25	39	25	39	30
CLSS, DTT	5.7	85	60	340	60	340	15