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Effect of CaM on the open conformation subpopulation of cross-linked CLSS
The reactions mixed each CaM-free or CaM-bound, pre-reduced enzyme with excess cytochrome c. The percentage open conformation were determined from the traces in Fig. 11. See text and “Experimental procedures” for details.
| Enzyme | Open % |
|
|---|---|---|
| −CaM | +CaM | |
| CLSS, pH9 | <1 | <1 |
| CLSS-BMH | 20.6 ± 1.9 | 30.6 ± 0.6 |
| CLSS-BM(PEG)2 | 39.2 ± 2.7 | 41.0 ± 1.1 |
| CLSS-BM(PEG)3 | 50.1 ± 0.3 | 52.0 ± 0.6 |
