Figure 4.
ASNase treatment promotes liver steatosis in obese wild type mice (Atf4f/f) and mice deleted for hepatic Atf4 (AlbCre Atf4f/f). A, body fat accumulation in male and female mice after 12 weeks of an obesogenic diet, assessed by magnetic resonance imaging. B, glucose clearance in male and female mice after 12 weeks of an obesogenic diet. C, total body weight loss after 8 days of ASNase or PBS. D, gross morphology of livers from lean and obese mice after 8 days of ASNase or PBS. Scale bar is 1 cm. E, triglyceride accumulation in livers of obese animals. F, hepatic ApoB protein abundance was assessed by immunoblotting. Left, representative picture; right, quantitative analysis of the ApoB100 band. Data are mean ± S.D. *, p < 0.05 as compared with respective PBS control. n.s., no significant differences.