Figure 6.
Genetic ablation of hepatic ATF4 fails to stimulate mTORC1 signaling during ASNase treatment. A, hepatic H2S production as measured by lead acetate method. B, quantitative analysis of Cth and Cbs gene expression, assessed using quantitative RT-PCR. C and D, immunoblot analysis for key components of mTORC1 pathway: representative images (C) and quantitative analysis of relative band intensities (D). Data are represented as mean ± S.D. *, p < 0.05 as compared with respective PBS control. # marks difference between strains (p < 0.05). Means not sharing a letter are different according to Tukey post hoc when drug x strain interaction was revealed by ANOVA. n.s., no significant differences.