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. 2017 Feb 14;292(16):6821–6837. doi: 10.1074/jbc.M116.770362

Figure 6.

Figure 6.

Br-PC quenching assay with Naa60 peptides indicate peripheral binding to Golgi-like liposomes. A, WT Naa60-(189–242) peptide (W227) and mutated Naa60-(189–242) peptides (Y214W/W227F, I209W/W227F, or Y193W/W227F) were incubated with Br-Golgi-like liposomes (52% Br-PC, 20% PE, 15% cholesterol, 8% PI(4)P, and 5% PS). Vesicles contained PC molecules carrying a quenching Br-group at either position (11,12), (9,10), (6,7), or (4,5) in the acyl chain. The fluorescence measured for vesicles binding to the brominated vesicles (F) was divided by the fluorescence for vesicles binding to the non-brominated vesicles (F0). n = 2 per peptide. B, based on data in A, the position of Trp residues relative to the center of the bilayer was calculated using the parallax method (34).