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. 2015 Nov 2;35(27):3514–3523. doi: 10.1038/onc.2015.412

Figure 3.

Figure 3

Functional interaction between Arkadia and ESRP2. (a) qRT–PCR analyses of the expression of Arkadia, ITGA6 exon 27, SLK exon 13 and TSC2 exon 27 in HEK293T cells in the presence of negative control siRNA (siNC) or siArkadia. Values were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH), total ITGA6, SLK and TSC2, respectively. (b) qRT–PCR analysis of Arkadia and ENAH exon 11a expression in OS-RC-2 cells in the presence of siNC or siArkadia. Values were normalized to GAPDH and total ENAH, respectively; n=3 in (a) and (b). *P<0.01 and **P<0.05 by two-sided Student's paired t-test. (c and d) Immunoprecipitation assay using HEK293T cells transiently transfected with ESRP1 (c) or ESRP2 (d) and Arkadia. IB, immunoblotting; IP, immunoprecipitation; WT, wild-type. (e) Immunoprecipitation assay using HEK293T cells to determine the interaction between endogenous ESRP2 and Arkadia proteins.