Figure 7.

miR-133a expression in vivo. (a) Metabolomics analysis of 44 species of diacylglycerols from soleus muscle excised from LF, or HFS, and a normal pregnancy (Lean Dam), or gestational diabetes (GDM Dam) during development, as indicated. Five highly abundant diacylglycerols are highlighted in the chart. Blue=low abundance, yellow=medium low abundance, orange=medium high abundance, red=high abundance. (b–e) Rat soleus muscle or heart tissue was excised and total RNA was extracted, from rodents treated as described in (a). qPCR analysis was performed using the ΔΔCT method, where RNU6 was used as an internal control for miR-133a, and β-actin was used as a control for PCG-1α and mitofusin-2. (f) Protein extracts from rat soleus muscle were subjected to immunoblot analysis, as indicated. PKCδ catalytic fragment (PKCδ c.f.). Data are represented as mean±S.E.M. *P<0.05 compared with control