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Loss of ATG5 enhances chaetocin-induced cell death. (a) Immunoblotting of autophagic markers (ATG5 and LC3-II) and apoptotic markers (PARP, cleaved caspase-3 and procaspase-3) from HepG2 cells transfected with the indicated siRNAs followed by chaetocin treatment for 16 h. (b) Cell viability assay on HepG2 cells following the treatments as described in (a). Shown are average of triplicate samples analyzed (n=3) and asterisks indicate P-value <0.005
