Figure 6.

Caspase-2-deficient mice are protected from liver injury induced by Western diet Aminotransferases (ALT, AST) and alkaline phosphatase (ALP) serum levels (a) in WT and caspase-2-deficient mice-fed chow (n=4/genotype) or Western diet (n=8/genotype) for 16 weeks. Representative liver sections stained with H&E (200x field) (b), TUNEL with quantification of number of positive cells (c), inflammatory markers F4/80 and YM-1 with morphometric quantification and qRT-PCR analysis in whole liver (d). Scale bar, 100 μm. The errors reported represent mean±S.E.M., normalized to expression in chow-diet-fed mice. *P<0.05, **P<0.01 chow versus Western diet. ^#P<0.05, ^##P<0.01 WT versus caspase-2-deficient mice. Interaction, assessed by two-way ANOVA, indicates that the effect of diet was different between genotypes