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. 2017 Apr 18;8:14909. doi: 10.1038/ncomms14909

Figure 2. LOX activity controls EGFR cell surface retention and activation.

Figure 2

(a) Schematic illustrating the secreted green fluorescent protein (sGFP, control), and various LOX-GFP expression constructs used in this study. SP, signal peptide; PP, pro-peptide; CD, catalytic domain; LOX, wild-type enzyme; K314A, catalytically inactive LOX mutant; ΔBMP-1; BMP-1 cleavage site-deleted LOX mutant. (b) Western blot showing immunoprecipitated sGFP and indicated LOX-GFP fusion proteins from MDA-MB-231 cell culture medium. (c) Western blots for pY1068 EGFR, surface EGFR, total EGFR, pAKT, total AKT and GAPDH in EGF-stimulated (3 min) control (siCtl) or LOX-depleted (siLOX #1) MDA-MB-231 cells expressing sGFP, and indicated LOX-GFP fusion proteins. (d) Quantification of experiments in c. Data are represented as mean±s.d. from four independent experiments. **P<0.01, Student's t-test.