Fig. 2.

Gel electrophoresis analysis of RT-LAMP products. a Testing RT-LAMP primers for Zika virus (ZV), chikungunya (CH), and dengue serotype 1 (D-1) in 1-plex and 3-plex formats. M is a 50 bp DNA ladder, NTC-z/c/d are no template controls for ZV, CH and D-1 primers. CH-m was total nucleic acid pre-extracted from CH-infected Ae. aegypti female mosquito. NSC was designated as non-specific control where total nucleic acid extracted from non-infected Ae. aegypti. Viral titers used were as follows: 2.85 pfu for ZV, 242 genomic copies for CH, and 1.22 pfu for D-1. b Testing different RT-LAMP temperatures (55 °C to 70 °C) and Mg²⁺ concentrations (4 mM to 10 mM) in the presence of all three LAMP primers for ZV, CH and D-1 with no target RNA