Fig. 4.

a Singleplex and multiplex detection of Zika (ZV) viral RNA (2.85 pfu) in 10% urine using 80 nM strand-displacing probe using Roche Light cycler (channel 483-533), b Singleplex and multiplex detection of chikungunya (CH) viral RNA (242 copies) in 10% urine using 80 nM strand-displacing probe using Roche Light cycler (channel 523-568), c Singleplex and multiplex detection of dengue-1 (D1) viral RNA (1.22 pfu) in 10% urine using 80 nM strand-displacing probe using Roche Light cycler (channel 558-610), d Fluorescent emission upon irradiation by a blue LED (470 nm) was visualized through orange filter glass. Each virus was assigned to a different fluorophore tag; FAM for Zika, HEX for chikungunya, and TAMRA for dengue