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. 2017 Apr 21;18:21. doi: 10.1186/s12860-017-0137-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Osteogenic differentiation on Collagen I-based Cell Carrier (CCC). a, b SEM images of differentiated DPPSC adhered on CCC surface (black arrow). c SEM image of differentiated DPPSC with hydroxyapatite deposition on CCC surface. d SEM image of hydroxyapatite deposition on CCC. Scale bars: 100 μm (a), 20 μm (b), 10 μm (c), 2 μm (d). e Microanalysis of the CCC surface with atomic concentrations. f RT-PCR gene expression analysis of differentiation markers (OC, ALP, COL1) and adhesion markers (VCAM1, VLA4) in DPPSC cultured on CCC, DPPSC cultured on 2D (plastic surface) and SAOS-2 cultured on CCC. GAPDH was used as a housekeeping. g, h Immunohistochemistry of differentiation markers (OC, OPN) in differentiated DPPSC on CCC. Scale bars: 1000 μm (g1, h1), 400 μm (g2, h2); 200 μm (g3, h3)