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. 2017 Apr 21;7:46559. doi: 10.1038/srep46559

Table 2. Cytopathic effects (CPE) observed in cell culture after five days.

  Day 1 Day 3 Day 6 Day 7 Day 14 Day 21
Sediment 7.5 g + + + + +
Sediment 25 g + + + + +
Sediment 50 g +
Sediment 100 g +
pH 4
pH 6 + +
pH 8 + + + + +
pH 10 + + + + + +
Salinity 0.05 g +
Salinity 1.0 g + + + + +
Salinity 3.0 g + + + + + +
Salinity 35.0 g + + + + +
Temperature 4 °C + + + + + +
Temperature 20 °C + + + + + +
Temperature 30 °C + + + + + +

Samples include distilled H2O (1 L) with treatments sediment (7.5, 25, 50, and 100 g/L), pH (4,6,8,10), salinity (0.05, 1.0, 3.0, and 35 g/L) and temperature (4, 20 and 30 °C) spiked with EHV-1 (103.5 TCID50/ml). Run over 21 days are sampled at six different points (days). +denotes positive for any CPE in cell culture. denotes negative for any CPE in cell culture.