Table 2. Cytopathic effects (CPE) observed in cell culture after five days.
| Day 1 | Day 3 | Day 6 | Day 7 | Day 14 | Day 21 | |
|---|---|---|---|---|---|---|
| Sediment 7.5 g | + | + | + | + | + | − |
| Sediment 25 g | + | + | + | + | + | − |
| Sediment 50 g | + | − | − | − | − | − |
| Sediment 100 g | + | − | − | − | − | − |
| pH 4 | − | − | − | − | − | − |
| pH 6 | + | + | − | − | − | − |
| pH 8 | + | + | + | + | + | − |
| pH 10 | + | + | + | + | + | + |
| Salinity 0.05 g | + | − | − | − | − | − |
| Salinity 1.0 g | + | + | + | + | + | − |
| Salinity 3.0 g | + | + | + | + | + | + |
| Salinity 35.0 g | + | + | + | + | + | − |
| Temperature 4 °C | + | + | + | + | + | + |
| Temperature 20 °C | + | + | + | + | + | + |
| Temperature 30 °C | + | + | + | + | + | + |
Samples include distilled H2O (1 L) with treatments sediment (7.5, 25, 50, and 100 g/L), pH (4,6,8,10), salinity (0.05, 1.0, 3.0, and 35 g/L) and temperature (4, 20 and 30 °C) spiked with EHV-1 (103.5 TCID50/ml). Run over 21 days are sampled at six different points (days). +denotes positive for any CPE in cell culture. −denotes negative for any CPE in cell culture.