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. 2017 Apr 21;7:46484. doi: 10.1038/srep46484

Table 2. Bacterial strains and plasmids used in this study.

Strain or plasmid Genotype or phenotype Source or reference
Strains
E. coli S17 RecA thi pro hsdR M+ RP4, SmR AmpR KanR This work
S. sanxanigenens NX02 Wild-type strain, Ss+, CmR This work
S. elodea ATCC31461 Wild-type strain, Gel+ This work
Sphingomonas sp. ATCC31555 Wild-type strain, Wel+ This work
Plasmids
pLO3 4937-bp suicide vector, tetR 53
pBBR1MCS-2 5144-bp broad host range vector, kanR 54
pLO3ssn pLO3 derivative carrying upstream and downstream fragment of ssn, “ssn” refers to all gene related to Ss biosynthesis This work
pBBRssB pBBR1MCS-2 derivative expressing ssB This work
pBBRssn pBBR1MCS-2 derivative expressing ssn, “ssn” refers to all related genes This work
pBBRSrssn pBBR1MCS-2 derivative expressing ssB and ssn simultaneously This work
pBBRSrgeln pBBR1MCS-2 derivative expressing ssB and geln simultaneously, “geln” refers to gelQ, gelL This work
pBBRgeln pBBR1MCS-2 derivative expressing geln, “geln” refers to gelD/gelCE/gelS/gelG This work
pBBRweln pBBR1MCS-2 derivative expressing weln, “weln” refers to welD/welCE/welS/welG This work