Strains |
E. coli S17 |
RecA thi pro hsdR− M+ RP4, SmR AmpR KanR
|
This work |
S. sanxanigenens NX02 |
Wild-type strain, Ss+, CmR
|
This work |
S. elodea ATCC31461 |
Wild-type strain, Gel+
|
This work |
Sphingomonas sp. ATCC31555 |
Wild-type strain, Wel+
|
This work |
Plasmids |
pLO3 |
4937-bp suicide vector, tetR
|
53 |
pBBR1MCS-2 |
5144-bp broad host range vector, kanR
|
54 |
pLO3ssn |
pLO3 derivative carrying upstream and downstream fragment of ssn, “ssn” refers to all gene related to Ss biosynthesis |
This work |
pBBRssB |
pBBR1MCS-2 derivative expressing ssB
|
This work |
pBBRssn |
pBBR1MCS-2 derivative expressing ssn, “ssn” refers to all related genes |
This work |
pBBRSrssn |
pBBR1MCS-2 derivative expressing ssB and ssn simultaneously |
This work |
pBBRSrgeln |
pBBR1MCS-2 derivative expressing ssB and geln simultaneously, “geln” refers to gelQ, gelL
|
This work |
pBBRgeln |
pBBR1MCS-2 derivative expressing geln, “geln” refers to gelD/gelCE/gelS/gelG
|
This work |
pBBRweln |
pBBR1MCS-2 derivative expressing weln, “weln” refers to welD/welCE/welS/welG
|
This work |