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. 2017 Apr 20;17:76. doi: 10.1186/s12870-017-1025-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Tapetal PCD during tobacco anther development by TUNEL assays. a–d Fluorescence microscope of cross-sections of tobacco anthers at stage 9 (a), stage 10 (b), stage 11 (c), and stage 12 (d). Green fluorescence indicates TUNEL-positive signals while red fluorescence indicates propidium iodide (PI) staining. Corresponding transmission images are placed below the fluorescence images. Bars, 50 μm