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. 2017 Apr 21;19:50. doi: 10.1186/s13058-017-0843-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Spheroid and ductal structures formed in collagen gels recapitulate a physiological breast bilayer. Expression of cytokeratin (CK) 8, P-cadherin, epithelial cell adhesion molecule (EpCAM) and vimentin spheroid (a) and ductal (b) structures formed from myoepithelial and luminal cells grown in collagen for 21 days. Representative images from sections of normal human breast ducts (a and b) are also presented. Cell nuclei are labelled with 4′,6-diamidino-2-phenylindole (blue). Scale bar = 20 μm. Images are representative of structures formed from cells derived from at least three donors