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. 2017 Apr 21;7:46719. doi: 10.1038/srep46719

Figure 4. Inhibition of SIV and MLV replication by opossum A1.

Figure 4

(a) Representative SIVmac single-cycle assays with varying concentrations (50 and 250 ng) of WT opossum A1 or deaminase-defective mutants (E62A or E62Q). Data represent the luciferase activity relative to vector control data (n = 3, average +/− SD with P values represented by asterisks). (b) Immunoblots of A1 in viral particles and cell lysates. A1 and SIVmac Gag were detected using anti-HA anti-p27 antibodies, respectively. β-actin was used a loading control. (c) Representative SIVagm single-cycle assays with varying concentrations (50 and 250 ng) of WT opossum A1 or deaminase-defective mutants. Data represent the luciferase activity relative to vector control data (n = 3, average +/− SD). P values represent comparisons with vector control data. (d) Representative MLV single-cycle assays with varying concentrations (60 and 300 ng) of WT opossum A1 or deaminase-defective mutants. Data represent the percentage of GFP+ cells relative to vector control data (n = 3, average +/− SD). P values represent comparisons with vector control data.