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. 2017 Apr 21;7:46719. doi: 10.1038/srep46719

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Representative L1_RP-GFP retrotransposition assays with varying concentrations (20, 100, or 500 ng) of WT opossum A1, or its catalytic mutants (E62A or E62Q). Retrotransposition frequency is presented relative to vector control data (n = 3, average +/− SD). P values represent comparisons with vector control data. (b) Immunoblotting of the indicated HA-tagged A1s expressed in HEK293T cells, with β-actin as the loading control. (c) A representative L1-neo retrotransposition assay with opossum A1 WT, or its catalytic mutants (E62A or E62Q). Retrotransposition frequency is presented relative to vector control data (n = 3, average +/− SD). P values represent comparisons with vector control data. (d) Immunoblotting of the indicated HA-tagged A1s expressed in HEK293T cells, with β-actin as the loading control.