Table 1.
Primers and probes used in this study.
| Target | Name | Oligonucleotide sequence (5′-3′) | Reference |
|---|---|---|---|
| pUC-cloning vectors | 401-F_PUC 18-F | TgT CgT gCC AgC TgC ATT A | (Mäde, Reiting, Strauch, Ketteritzsch, & Wicke, 2008) |
| 401-R_pUC 18-R | gAg CgA ggA AgC ggA AgA g | ||
| 401-Tex_Tm-Puc18 | TexasRed–AAT Cgg CCA ACg CgC gg-BHQ2 | ||
| IPC-fw | TgT gAA ATA CCg CAC AgA Tg | (Messelhäusser et al., 2007) | |
| IPC-re | AgC Tgg CgT AAT AgC gAA G | ||
| IPC-S | HEX-gAg AAA ATA CCg CAT CAg gC-TAMRA | ||
| Chloramphenicol acetyl transferase gene (cat) | 356-F_Cat-Staph-F | CAg CTT TTA gAA CTg gTT ACA ATA gCg | This work |
| 356-R_Cat-Staph-R | gCA TgA TAA CCA TCA CAA ACA gAA T | ||
| Plasmid pAM-beta1 (ermAM adenine methylase gene -) | 357-F2 | CgT CTA TTg AAT TAg ACA gTC ATC TAT TCA | This work |
| 357-R2 | Tgg AAC ATC TgT ggT ATg gCg | ||
| Integration site of cat in B. subtilis isolate e871 | 558-F | CgA gCT TTT gCg CgT ATA | This work |
| 558-R | gCC ATT CCA ATA CAA AAC CAC ATA | ||
| 558-Tm | FAM-Cgg ATC TAA CgC ATg CTC CgC A-BBQ | ||
| Plasmid pGMBsub01 (junction of pUB110 to pUC19) | 690-F | gAT gAA TTA TAT CAA CAT ATT AAg CCT TTg g | This work |
| 690-R | gCT Atg ACC ATg ATT Acg CCA Ag | ||
| 690-Yak | Yak-AAg ATC Cgg ggA ATT gCT gCA gg-BBQ | ||
| Plasmid pGMBsub01 (junction of B. amyloliquefaciens rib-operon to pUC19) | 691-F | CgA TTA AgT Tgg gTA ACg CCA | This work |
| 691-R | TTC TCT AAA gAA AAC TgC TCg TAC g | ||
| 691-Tm | FAM-ACg gCC AgT gAA TTC gCA AgA Cg-BBQ | ||
| Plasmid pGMBsub02 (junction of deleted B. amyloliquefaciens rib-operon) | 804-F1 | AgA CCg CgT TTA Cag TCA gCA T | This work |
| 804-R1 | CTCg AAT TCT TTT TTC gTT CCA A | ||
| 804-Tm | FAM-ACC ACA AgC TgA CCg AAT ATg Cgg AT-BBQ | ||
| Plasmid pGMBsub03 (junction of 3′-rib-fragment to pUC19) | 693-F | TCgTgCACAgCTTgAAATCTAgA | This work |
| 693-R | ggA AAC AgC TAT gAC CAT gAT TAC g | ||
| Cy5-693 | Cy5-CCT CTA gAg TCg ACC TgC Agg CAT gC-BBQ | ||
| Plasmid pGMBsub04 (junction of rib-operon-fragment to pSM19035) | 694-F | CAT TCg ATT gTg CgA gCg | This work |
| 694-R | Tgg TAT TTT TTg TAT TCA gCg TAA Cag ACA TAA T | ||
| 694-Tm | FAM-Cag gCg AAT TCC AgT TAA ATT CCg TgT Agg--BBQ | ||