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. Author manuscript; available in PMC: 2017 Apr 21.
Published in final edited form as: Oncogene. 2009 Sep 14;28(46):4095–4104. doi: 10.1038/onc.2009.273

Figure 2.

Figure 2

Menin inhibits A549 lung cancer cell proliferation partly through down-regulating PTN expression. (A) A549 cells were stably transfected with either empty vector or vector expressing one of three distinct PTN shRNAs. The efficiency of PTN knockdown was determined by measuring the secreted PTN by ELISA. (B) The cells generated above were evaluated for their proliferation at indicated on days of culture. (C) Growth curve of A549 cells stably transfected with either empty vector (pcDNA3.1) or pcDNA3.1-PTN. (D) A549 cells were stably transfected with either vector or vector expressing either menin or PTN, or cotransfected with menin and PTN-expressing constructs. (E,F) The over-expression of menin and its effect on down-regulation on PTN. * p<0.05 vs. control group.