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. 2017 Mar 16;6(4):518–524. doi: 10.1242/bio.023671

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Effect of continuous tensile strain on the morphology of HPL cells. (A-D) Images of the PDL stained with H&E stain. Control group (A and B) and 5 days after tooth movement (C and D). B and D are higher magnification images of the boxed area in A and C. Arrow indicates the direction of tooth movement. B: bone, D: dentin. Scale bars: 100 μm. The effect of continuous tensile strain on cell morphology and cytoskeletal was assessed by means of F-actin staining. (E,F) The green fluorescence indicates the F-actin. Representative photographs of the control (E) and stretched HPL cells (F) are shown. Scale bars: 50 μm. (G) The effect of continuous tensile strain from the device on cell viability examined by using cell counting kit-8 (E). Percentage of controls is shown. Mean±s.d.; NS, not significant.