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. 2017 Mar 16;6(4):518–524. doi: 10.1242/bio.023671

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Details of Cell Extender (ver. 3). (A) Design of Cell Extender (ver. 3). The device was composed of BioFlex^® plate, Cell Extender device, AC adaptor, and controller with LCD display. The size of Cell Extender device is 90×130×40 mm. (B) The Cell Extender device (top) and BioFlex^® plate (middle) with the 15-cm scale (bottom). (C) BioFlex^® plate positioned on the loading platform of Cell Extender device. (D) Schematic illustration of the flexible membranes at the bottom of BioFlex^® plate. When the screw (red) moves upwards, it deforms the flexible membrane of BioFlex^® plate (green) and applies strain to the cells on the flexible membrane. Arrow indicates the direction of movement of the screw, and yellow double-arrow indicates the direction of tensile force. (E) Schematic illustration which shows the factors for calculating formula of deformation. x_s: screw extension, r_s: screw radius, r_d+r_s: radius of well bottom, and r_d′: change in extension.