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. 2017 Feb 22;6(4):463–470. doi: 10.1242/bio.023952

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Xl_Kif18A is important for meiotic spindle structure. (A) Scheme of the depletion /add-back experiments. (B) Immunoblot of control (IgG)- or Kif18A (Ab^18Apep)-depleted extracts supplemented with mRNA encoding wt, C_i or Δtail Flag-eGFP-Xl_Kif18A. Right panel shows immunoblot of IgG or Ab^18Apep beads. (C) Representative fluorescence images of spindles obtained as described in A. DNA, αβ-tubulin, and Flag-eGFP-Xl_Kif18A are shown in blue, red and green, respectively. Scale bar: 10 µm. (D) Quantification of spindle length to width ratio. (E) Quantification of spindles with multiple/unfocused poles (more than 60 spindles analyzed per condition, mean±s.d., unpaired t-test: ****P≤0.0001).