Fig. 4.

Xl_Kif18A can complement the function of human Kif18A. (A) Scheme of the RNAi/rescue experiments using HeLa cells expressing constitutively CENP-A-mCherry and inducibly siRNA resistant human or Xenopus eGFP-Kif18A. (B) Quantification of mitotic timing (timing from NEBD to either commitment to anaphase onset or apoptosis; top) in cell lines treated as described in A using fluorescent time-lapse imaging (time resolution 5 min, more than 200 cells per condition, mean±s.d. in red, n=3 independent experiments) and immunoblot analyses (bottom) of respective cells. Unspecific band is marked with an asterisk. (C) Representative fluorescence images of spindles in mitotic cells expressing eGFP-Kif18A constructs as indicated. HURP, CENP-A-mCherry, and eGFP-Kif18A are shown in blue, red and green, respectively. Insets show magnified view of area marked with white box. Scale bar: 10 µm.