Fig. 3.

Respiration and ATP synthesis in isolated mitochondria. (A) Rate of oxygen consumption measured using NADH as an electron donor, alone (NADH, state 4), after further addition of ADP (NADH+ADP, state 3) or CCCP (NADH+CCCP, uncoupled respiration). (B) Rate of oxygen consumption measured using ascorbate/TMPD as an electron source in the presence of CCCP (Asc/TMPD+CCCP). (C) Rates of ATP synthesis measured using NADH as a respiratory substrate and in the presence of a large excess of external ADP. Mitochondria were prepared from cells grown as described in Fig. 2B. Statistical analysis was done with Tukey's test (*P<0.05; **P<0.01; ***P<0.001; ****P<0.0001). Data are mean±s.d. (n=4).