Fig. 4.

H4K5 acetylation is reduced by activation of HDAC3 activity using theophylline and H4K5 acetylation increased by HDAC3 inhibition with RGFP966 treatment in myogenic progenitors. H4K5ac is the main target of HDAC3 and was used to determine HDAC3 activity in cells. (A,B) Western blotting of whole cell lysates treated with theophylline (A) or RGFP966 (B) to analyze H4K5 acetylation in differentiating wild-type or emerin-null progenitors. Three biological replicates are shown for each treatment. (C,D) Densitometry was performed and H4K5ac protein levels in each sample were normalized to total H4 levels in each sample. Levels of H4K5ac in each treatment condition were then normalized to DMSO-treated wild-type cells. Results are mean±s.d. of n=3 for each condition; *P<0.05, **P<0.01 using unpaired, two-tailed t-tests.