Fig. 6.

ERK inhibition by U0126 treatment rescued MyHC expression and myotube formation in differentiating emerin-null progenitors. (A) Timeline showing the timing of U0126 addition and sample collection for western blot analysis of whole cell lysates during differentiation. (B-I′) Representative images of vehicle-treated wild-type (B-E) or emerin-null (F-I) cells and U0126-treated wild-type (B′-E′) or emerin-null (F′-I′) cells 36 h after differentiation induction. 40× magnification. (J-L) Quantification of >1000 nuclei for each experimental treatment (n≥3) was done to determine the percentage of myogenic progenitors in the cell cycle (J), percentage of cells expressing MyHC (K) and the number of myotubes formed (L) 36 h post-differentiation induction. Results are mean±s.d. of n≥3; N.S., not significant; *P<0.05 using paired, two-tailed t-tests.