Figure 4.

(A) Histology of the specimens after 4, 8, and 12 weeks after implantation in vivo. Hematoxylin and eosin staining of harvested tissues in CS/HA, CS-5%P24/HA, and CS-10%P24/HA groups. Red arrows show new formed blood vessels in or around the scaffolds. (B) Immunohistology. Cells or area in dark brown represent OCN-positive cells, and the areas in white are voids. (C) Immunohistology. Area in dark brown is positive area of CD31, indicating the blood vessels, and the areas in white are voids. The representative images at 10× magnification and at 40× magnification are presented. (At 10× magnification, scale bar = 100 μm. At 40× magnification, scale bar = 50 μm.)