Figure 1.

High affinity binding of aptamer HL-1 to Maver-1 lymphoma cells. (A) Sequences of the developed aptamers HL-1 and HL-2. (B) Predicted secondary structures of aptamer sequences. (C) High affinity binding of aptamers to Maver-1 lymphoma cells with no reaction to off-target control Jeko-1 cells. Cultured cells were treated with Cy-3 labeled aptamers at different final concentrations, and the resultant cell binding was quantified by flow cytometry. (D) Specificities of aptamers were validated at 20 nmol/L in multiple cultured cell lines with anti-lambda light chain antibody as a control. Cell binding was examined by flow cytometry. (E) Function characterization of aptamer sequences. Aptamer HL-1 was truncated and tested with or without primer region(s) and/or an intact core. Binding of each truncated sequence to Maver-1 lymphoma cells at 50 nmol/L final concentration was quantified by flow cytometry. Relative cell binding capacities (%) of truncated aptamers were normalized to the full-length aptamer HL-1 (used as baseline).