Skip to main content
. 2017 Mar 6;7(5):1214–1224. doi: 10.7150/thno.17252

Figure 6.

Figure 6

Effects of autophagy inhibitors on the 45 nm AuNP-induced osteogenic differentiation. PDLPs were treated with 45 nm AuNPs (10 μM) alone, or combine with 3-MA (2 mM) or CQ (5 μM). (A) ALP staining on day 7, alizarin red S and von Kossa staining on day 21. (B) Real-time PCR analysis of Runx2, ALP, COL1, OPN, and OCN mRNA expression on day 5. (C) Western blot analysis of Runx2, ALP, COL1 and OPN protein expression on day 7. P values for the comparison with control were selectively shown. *p<0.05, **p<0.01.