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. 2017 Mar 6;7(5):1245–1256. doi: 10.7150/thno.18187

Figure 2.

Figure 2

Fluorescence response to caspase-3. (a) Fluorescence emission spectra of FRET-Pep incubated with 4.0 U mL-1 caspase-3 for 0, 10, 20, 40, 60, 80 and 120 min. (b) Plots of fluorescence intensity at 520 nm vs. incubation time of FRET-Pep with 4.0 U mL-1 caspase-3. (c) Fluorescence emission spectra of FRET-Pep after incubation with 0, 0.5, 1.5, 2.5, 4.0 and 6.0 U mL-1 caspase-3 for 60 min. (d) Plot of fluorescence intensity at 520 nm vs. caspase-3 concentration. Fluorescence emission spectral response of GAI@CP to 4.0 U mL-1 caspase-3 (e) at pH 5.0/7.4 and (f) in the absence or presence of 80 μM Z-DEVD-FMK as caspase-3 inhibitor at pH 5.0 after incubation for 24 h.