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. 2017 Mar 6;7(5):1245–1256. doi: 10.7150/thno.18187

Figure 3.

Figure 3

In vitro targeted drug delivery, rapid endo/lysosomal escape and therapeutic self-reporting. (a) MTT assays for HeLa cells incubated with GAI@CP and GI@CP for 24 h, and FA block group was pre-blocked by FA for 30 min and then incubated with GAI@CP for 24 h. (b) MTT assays for HeLa, A549 and HUVEC cells incubated with GAI@CP for 24 h. (c) Confocal fluorescence images of AO staining to study lysosomal disruption of HeLa cells treated with PBS as control, GA@CP and GAI@CP, respectively. Scale bars: 10 μm. (d) MTT assays for HeLa cells incubated with CPT, GA@CP and GAI@CP for different times as indicated. Data are means ± SD (n ≥ 3, *P < 0.05, **P < 0.01 using a one-way ANOVA). (e) Confocal fluorescence images of HeLa cells stained with PI after incubated with GAI@CP and GAI@C for 24 h. Scale bars: 10 μm. (f) Flow cytometric assays of HeLa cells treated with GAI@CP, GA@CP, GI@CP and GAI@P for 24 h, respectively.