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. 2017 Mar 22;7(5):1360–1372. doi: 10.7150/thno.16532

Figure 3.

Figure 3

Nucleolin-dependent tumor targeting of modified EVs in vitro. A. Flow cytometry analysis of nucleolin expression on the surface of MDA-MB231 and C2C12 cells. B. Uptake of AS1411-EVs-let-7-Cy3 detected by flow cytometry in C2C12 and MDA-MB-231 cells. AS1411-EVs or C-AS1411-EVs (control group in MDA-MB-231) loaded with let-7-Cy3 were incubated with the cells at 37 °C for 1 h. Anti-nucleolin antibody was also used in MDA-MB-231 cells for blocking the target-specific binding. The fluorescent signal was relatively stronger in the MDA-MB-231 cells incubated with AS1411-EVs-let-7-Cy3. C. Cell-associated AS1411-EVs-let-7-Cy3 was detected in MDA-MB-231 cells using fluorescence microscopy. Cells were treated with PBS then co-cultured for 1 h with AS1411-EVs, AS1411-EVs-let-7-Cy3 or C-AS1411-EVs-let-7-Cy3. Cells were also treated with anti-nucleolin antibody then co-cultured for 1 h with AS1411-EVs-let-7-Cy3. (Scale bar = 100 μm).