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. 2017 Mar 23;7(5):1389–1406. doi: 10.7150/thno.17647

Figure 6.

Figure 6

Resveratrol inhibits mitochondrial fission and induces the suppression of NPC stemness by reducing the mitochondrial localization of COX-2 and p53. (A-B) CNE1 and CNE2 cells were treated with or without 100 μM resveratrol (RSV) for 24 h. (A) Confocal microscopy images of CNE1 and CNE2 cells immunostained with anti-COX-2 (green), anti-p53 (blue) antibodies, and MitoTracker (red). Manders' overlap coefficients for the co-localization of COX-2-mitochondria, p53-mitochondria, and COX-2-p53 are shown. Mitochondrial fragmentation counts were calculated by IPP6.0 and shown in bar graphs. (B) Whole cell lysates (WCL) and mitochondrial (Mito) and cytosolic (Cyto) fractions of CNE1 and CNE2 cells with or without treatment were prepared. WB assays were performed to determine the expression levels of COX-2, p-Drp1Ser616, p-Drp1Ser637, t-Drp1 in WCL and those of COX-2, p53, p-Drp1 Ser616 in Mito and Cyto fractions. (C-D) CNE1 and CNE2 cells treated with 0, 50, 100 μM RSV for 24 h were harvested. (C) COX-2, ABCG2 and Oct4 protein level was determined by WB assays. (D) The proportion of SP cells was analyzed by FCM. (E) Quantification of SP cells in CNE1 and CNE2 was shown in bar graphs. * P < 0.05, compared with the Ctrl group.