Skip to main content
. 2017 Feb 21;66(5):1293–1300. doi: 10.2337/db16-0887

Figure 2.

Figure 2

Insulin-positive cells are produced from PP cells upon coexpression of Mafa and Pdx1 in pancreatic endocrine precursor cells. A: Immunohistochemical analysis of pancreata from Ngn3-Cre, Ngn3-Cre;CAT-Mafa, Ngn3-Cre;CAT-Pdx1, and Ngn3-Cre;CAT-Mafa;CAT-Pdx1 mice at P0.5 and 6 weeks for insulin, PP, and somatostatin (Sst). Nuclei were stained with DAPI. There were PP cells coexpressing PP and insulin at P0.5 and 6 weeks. Arrows depict insulin and PP–copositive cells. Scale bars: 50 μm. B: The ratio of insulin and somatostatin–positive cells to somatostatin-positive cells was compared at P0.5; the somatostatin-positive cell–to–insulin-positive cell ratio was compared at 6 weeks. n = 3–6. 6W, 6 weeks; INS, insulin. *P < 0.001.