Figure 4.

Mast cell CM from cold-shocked mast cells induces UCP1 expression in adipocytes. Mast cell CM from Fig. 3 was applied to differentiated 3T3-L1 adipocytes for 4 h at 37°C. A and B: Adipocyte gene expression was measured for UCP1 and IL-4. C: Differentiated 3T3-L1 adipocytes were treated with recombinant mouse IL-4 (1 nmol/L), histamine (10 nmol/L), or a combination of both for 3 h. Data are mean ± SEM (n = 3). *P < 0.05, **P < 0.01 (ANOVA with Dunnett post hoc test).