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. 2017 Apr 1;144(7):1261–1272. doi: 10.1242/dev.140723

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Abdominal body muscles develop under mechanical tension. (A-B″) Time points from spinning disc confocal movies of myotubes labeled by Mef2-GAL4, UAS-GFP-Gma at 36 h and 40 h APF before (A,B) and after (A′,A″,B′,B″) complete myotube severing using laser cutting (Movies 3, 4). Newly created myotube ends (orange arrowheads) move away from the cutting site (marked by ‘+’ in A,B). Anterior and posterior myotube ends move outwards; compare pre-cut (green arrowheads in A,B) with post-cut (blue arrowheads in A′, B′) ends. (A‴,B‴) Kymographs of Movies 3 and 4 displaying intensities at the red lines indicated in A and B. (C,D) Schemata of the laser cuts; myotube movement after laser severing is indicated by arrows. Scale bar: 10 µm.