Fig. 5.

eEF1A mRNA depletion in the PGCs depends on its PRE. (A-D) Fertilized eggs were injected with either a control morpholino or a morpholino targeting the eEF1A PRE. Embryos were fixed at blastula stage for eEF1A in situ hybridization (red) followed by an immunofluorescence using Vasa antibody (green). Approximately 100 embryos were visualized and representative embryos are presented. Arrows indicate the PGCs. Scale bar: 20 µm. (E) The ratio of eEF1A mRNA present in the PGCs versus the somatic cells was quantified using 10 blastulae for the control morpholino and 12 for the PRE morpholino, and presented as percentages. Significance was assessed between the control and the PRE morpholino using Student's t-test (*P<0.005).