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. 2017 Apr 1;144(7):1273–1282. doi: 10.1242/dev.145722

Fig. 5.

Fig. 5.

SET-4 promotes dauer arrest by repressing the X-linked ins-9 gene. (A) ins-9 is repressed by DPY-21, SET-4 and DAF-16/FoxO but not DAF-12. Results are the mean±s.e.m. of five experiments. (B) ins-9 overexpression suppresses dauer arrest in eak-7;akt-1 animals. Non-transgenic (NT) and transgenic (T) progeny of transgenic hermaphrodites are shown. n=1200, 1522, 718/156 (NT/T), 702/210 (NT/T). (C) An ins-9::SL2::mNG transgene is expressed specifically in ASI sensory neurons. The arrowhead indicates the top of the nerve ring; the arrow indicates the ASI axon. Representative images are shown (n=20). (D) Two ins-9 nonsense mutations partially rescue dauer arrest in set-4;daf-2 mutants. n=957, 1205, 1398 (left); n=892, 1028, 897 (right). (E) ins-9 and akt-2 mutations rescue dauer arrest in an additive fashion in set-4;daf-2 mutants (n=949, 1281, 1334, 1524, 1333, 1478). (F) ins-9 and ins-7 mutations rescue dauer arrest in an additive fashion in set-4;daf-2 mutants (n=1396, 1251, 754, 827, 1327). *P<0.05, **P<0.01.