Fig. 3.

AEE788 prevents basal body duplication. Anti-TbSAS6 and YL1/2 were used to quantitate the number of mBBs and immature pBBs per trypanosome after treatment with AEE788 (5 µM) or DMSO (0.1%). (A) Representative staining pattern of YL1/2 (red) and anti-TbSAS6 (green) after DMSO (top) or AEE788 (bottom) treatment. Cells are counterstained with DAPI (1.5 µM). Red arrows indicate TbRP2⁺ foci (mBB), green arrows indicate TbSAS6⁺ foci (pBB), and yellow arrows indicate colocalization of TbRP2 and TbSAS6 (mBB). Scale bar, 6 µm. The basal bodies and associated kinetoplast (K) are enlarged in a single trypanosome for both treatment groups: DMSO (yellow boxes) and AEE788 (orange boxes). (B) Average percentage of trypanosomes (n = 125) with the indicated number of mBBs and pBBs after treatment with AEE788 or DMSO. Error bars represent the S.D. in three independent experiments. Statistical significance of changes in the distribution of the number of basal bodies (per cell) in the trypanosome population was determined with a Pearson χ² test (p = 0.3 × 10⁻¹⁸).