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Extended AEE788 exposure decreases trypanosome viability. Trypanosomes were treated with AEE788 (5 µM) or DMSO (0.1%) for 4, 9, or 16 hours, harvested, and treated with PI (3 µM) prior to analysis on a flow cytometer. Trypanosomes were gated based on size and shape (forward and side scatter) and the intensity of PI determined.
