TABLE 1.
Select examples of phosphoproteins affected by short-term (4 h) AEE8788 treatment
After treatment of trypanosomes with DMSO (0.1%) or AEE788 (5 µM) for 4 h, peptides were harvested and phosphopeptides were enriched over an IMAC column. LC-MS/MS was used to monitor the abundance of phosphopeptides in three independent experiments. Spectral counts indicate the combined number of times a phosphopeptide was observed over all experiments. The number in parenthesis indicates the total number of peptides observed for the parent protein over all experiments (summation of all peptides observed in the IMAC elution and flow through). (Taus et al., 2011) probability ≥80%]. A Student’s t test was used to determine whether the change in phosphopeptide abundance was statistically significant (P < 0.05).
| Gene ID | Production Description | Identified Phosphopeptide | Spectral Counts |
P Value | |
|---|---|---|---|---|---|
| DMSO | AEE788 | ||||
| Decreased | |||||
| Tb427.06.4970 | SRPK | HsASTNGPSQPAHQR | 6 (15) | 1 (3) | 0.038 |
| Tb427.10.3010 | Bilobe protein | sRISTGISFLSK | 5 (18) | 0 (7) | 0.038 |
| Tb427tmp.02.0810 | TbSAS4 | LAVGDANHSESIGDKSVstK | 8 (12) | 2 (3) | 0.013 |
| Increased | |||||
| Tb427.01.2100 | Calpain-like cysteine peptidase | AEEASPAPSPAGEsDEKAsKSEHESEAK | 20 (88) | 44 (99) | 0.03 |
SRPK, serine-arginine protein kinase.