TABLE 2.
Select examples of phosphoproteins affected by long-term (9-h) AEE8788 treatment
After treatment of trypanosomes with DMSO (0.1%) or AEE788 (5 µM) for 4 h, trypanosome phosphopeptides were enriched over an IMAC column. Phosphopeptide abundance was monitored by LC-MS/MS in three independent experiments. Spectral counts indicate the combined number of times a phosphopeptide was observed over all three experiments. The number in parenthesis indicates the total number of peptides observed for the parent protein over all experiments (summation of all peptides observed in the IMAC elution and flow through). [phosphoRS (Taus et al., 2011) probability ≥80%]. A Student’s t test was used to determine whether the change in phosphopeptide abundance was statistically significant (P < 0.05).
| Gene ID | Production Description | Identified Phosphopeptide | Spectral Counts |
P Value | |
|---|---|---|---|---|---|
| DMSO | AEE788 | ||||
| Decreased | |||||
| Tb427.03.3080 | NEK | ADTsDIsLSHEDLsR | 12 (16) | 0 (7) | 0.02 |
| Tb427.10.14010 | TbRP2 | EATPPEsASRSDSSAPTTPHSR | 8 (25) | 1 (7) | 0.05 |
| Tb427.10.8820 | Bilobe protein | TIGTTSGHSTTNLssHTPEK | 6 (17) | 0 (5) | 0.03 |
| Tb427tmp.01.3960 | BILBO-1 | LMSEASsFLGNLR | 5 (41) | 0 (26) | 0.04 |
| Increased | |||||
| Tb427.10.14770 | Associated kinase of Tb14-3-3 | LANSsLPVsHTSTR | 7 (13) | 15 (18) | 0.02 |
| Tb427.07.7000 | Bilobe protein | TSSHIsEHGLDR | 0 (38) | 10 (67) | 0.02 |
| Tb427.04.310 | Ubiquitin-transferase | TTLSKsAHVsHER | 3 (3) | 8 (9) | 0.04 |
NEK, NIMA (never in mitosis gene a)–related kinase.